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KMID : 0880220090470020178
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Journal of Microbiology
2009 Volume.47 No. 2 p.178 ~ p.186
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Intracellular Substrates of a Heme-Containing Ascorbate Oxidase in Pleurotus ostreatus
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Lee Seung-Rock
Kang Sa-Ouk
Joo Woo-Jeong
Baek Yong-Un
Lee Youn-Kyong
Yu Seong-Woon
Kim Yeon-Ran
Chay Kee-Oh
Cho Seung-Hyun
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Abstract
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A novel heme-containing ascorbate oxidase isolated from oyster mushroom, Pleurotus ostreatus, catalyzes oxidation of ascorbic acid (Kim et al., 1996). In this report, we describe the identification of intracellular substrates of the enzyme in the mushroom. Six compounds, which can serve as substrate of the heme-containing ascorbate oxidase, were identified as L-ascorbic acid, D-erythroascorbic acid, 5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, 5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid, 5-methyl-5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, and 5-methyl-5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid. All of the compounds were oxidized at a significant rate by the heme-containing ascorbate oxidase. Oxidation of the compounds produced equimolar amounts of hydrogen peroxide per mole of substrate.
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KEYWORD
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ascorbate oxidase, substrates, Pleurotus ostreatus
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